Аннотация
Список литературы
В настоящее время единственным методом, позволяющим заместить все функции печени, является органная трансплантация. Ввиду нехватки донорских органов, для временного замещения различных функций печени разрабатываются различные модели биоискусственной печени. Децеллюляризация непригодной для трансплантации печени от умершего донора является актуальной темой исследования.Приведены данные о технических особенностях подготовки печени к процессу децеллюляризации. Описан алгоритм получения бесклеточного матрикса печени человека и процесс проведения децеллюризации с помощью устройства для динамической консервации донорских органов с последующей оценкой методом электронной микроскопии.
Ключевые слова:
: децеллюризация печени, машинная перфузия, «скаффолд»
MACHINE PERFUSION FOR DECELLULARIZATION
Currently, the only method that can replace all the liver functions is organ transplantation. Due to the donor organs shortage, various bioartificial liver models are beingdeveloped. Decellularization of a liver unsuitable for transplantation from a deceaseddonor is a challenging topic of research. Data on the technical features of the preparationof the liver for the process of decellularization are given. An algorithm for obtaininga cell-free matrix of the human liver and the process of decellularization using a devicefor dynamic conservation of donor organs with subsequent evaluation by electron microscopyare described.
Keywords
decellularization of the liver, machine perfusion, human liver scaffold
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